将大鼠结肠黏膜解离为单个上皮细胞并进行显微镜观察。
Dissociation of rat colonic mucosa into single epithelial cells and their microscopic visualization.
作者信息
Herp A, Perret V, Suba E
出版信息
Microsc Acta. 1979 Nov;82(3):251-3.
PMID:530095
Abstract
Repeated incubation of rat colonic mucosa with phosphate-buffered mannitol yields morphologically intact single cell suspensions which are easily visualized microscopically with trypan blue. Cell dimensions are determined by means of a Nikon microcomparator.
摘要
用磷酸盐缓冲的甘露醇反复孵育大鼠结肠黏膜，可得到形态完整的单细胞悬液，用台盼蓝在显微镜下很容易观察到。细胞尺寸通过尼康显微比较仪测定。
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