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Dissociation of rat colonic mucosa into single epithelial cells and their microscopic visualization.

作者信息

Herp A, Perret V, Suba E

出版信息

Microsc Acta. 1979 Nov;82(3):251-3.

PMID:530095
Abstract

Repeated incubation of rat colonic mucosa with phosphate-buffered mannitol yields morphologically intact single cell suspensions which are easily visualized microscopically with trypan blue. Cell dimensions are determined by means of a Nikon microcomparator.

摘要

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