用从烟草叶片中分离出的膜脂和聚鸟氨酸对烟草花叶病毒进行拆解。

Disassembly of tobacco mosaic virus by membrane lipid isolated from tobacco leaves and polyornithine.

作者信息

Kiho Y, Abe T, Ohashi Y

出版信息

Microbiol Immunol. 1979;23(11):1067-76. doi: 10.1111/j.1348-0421.1979.tb00538.x.

DOI:10.1111/j.1348-0421.1979.tb00538.x

Abstract

In vitro disassembly of tobacco mosaic virus (TMV) virions occurred in the presence of both polyornithine and a lipid fraction isolated from tobacco leaf membrane. The latter could be replaced by lecithine. Disassembly of 10 microgram of TMV virions was attained in the presence of a 500-mg leaf equivalent of membrane lipid and 20 microgram of polyornithine in 1 ml of 0.01 M Tris-HCl buffer, pH 7.4 at 30 C. Similarity and dissimilarity between the in vitro disassembly and the in vivo uncoating mechanisms are discussed.

摘要

在多聚鸟氨酸和从烟草叶片膜中分离出的脂质部分存在的情况下，烟草花叶病毒（TMV）病毒粒子会在体外发生解体。后者可用卵磷脂替代。在含有相当于500毫克叶片的膜脂和20微克多聚鸟氨酸的1毫升0.01M Tris-HCl缓冲液（pH 7.4）中，于30℃下，10微克TMV病毒粒子会发生解体。文中还讨论了体外解体与体内脱壳机制之间的异同。