Patrono C, Wennmalm A, Ciabattoni G, Nowak J, Pugliese F, Cinotti G A
Prostaglandins. 1979 Oct;18(4):623-9. doi: 10.1016/0090-6980(79)90029-7.
In order to verify the validity of the assumption that male urinary Prostaglandin (PG) E2 reflects its renal production, PGE2 and PGF2 alpha concentrations were measured by radioimmunoassay in the renal venous plasma (RVP) and urine (U) of 12 male and 4 female healthy volunteers. While women had a similar PGE2/PFG2 alpha ratio in RVP (0.59 +/- 0.18) and U (0.41 + 0.06), men and a significantly (P less than 0.05) higher ratio in U (1.43 +/- 1.72) as compared to RVP (0.54 +/- 0.16). This was largely due to considerably higher and more variable U-PGE1 concentrations (roughly 6 times higher than female values), despite almost identical RVP levels. The possibility of an increased U excretion of a cross-reacting member of the PG-system, as a cause of such apparently high PGE2-like immunoreactivity (LI), was ruled out by TLC characterization of PGE2-LI with three different anti-PGE1 sera. Thus, male U-PGE2 may variably reflect an extra-renal source, such as contamination with trace amounts of seminal fluid. It is concluded that, unless such a contamination can be monitored and corrected for, measurement of male U-PGE1 should be considered of questionable relevance to renal PG-synthesis.
为了验证男性尿液中前列腺素（PG）E2反映其肾脏生成这一假设的有效性，采用放射免疫分析法对12名男性和4名女性健康志愿者的肾静脉血浆（RVP）和尿液（U）中的PGE2和PGF2α浓度进行了测定。女性的RVP（0.59±0.18）和U（0.41 + 0.06）中的PGE2/PFG2α比值相似，而男性U中的该比值（1.43±1.72）显著高于（P<0.05）RVP中的比值（0.54±0.16）。这主要是由于U-PGE1浓度显著更高且变化更大（大约比女性值高6倍），尽管RVP水平几乎相同。通过用三种不同的抗PGE1血清对PGE2-LI进行薄层色谱表征，排除了PG系统中交叉反应成员的U排泄增加作为这种明显高的PGE2样免疫反应性（LI）原因的可能性。因此，男性U-PGE2可能不同程度地反映肾外来源，如微量精液污染。结论是，除非能够监测并校正这种污染，否则男性U-PGE1的测量对于肾脏PG合成的相关性应被视为存在疑问。
