Stubblefield E
Chromosoma. 1975 Dec 10;53(3):209-21. doi: 10.1007/BF00329172.
Chinese hamster fibroblasts were synchronized and given 5-bromodeoxyuridine for DNA synthesis except during one hour of the S phase when thymidine was present in the medium. In the next mitosis, chromosomes stained with 33258 Hoechst were banded in appearance when photographed by fluorescence microscopy. The bright regions corresponded to the chromosome segments replicated during the thymidine exposure in the S phase. The segments replicated together during any one hour produced three distinct patterns which were characteristic of early, middle, and late S phase. Most of the fluorescent regions corresponded in size and position with G-bands of these chromosomes. There was no correlation between the staining behavior of a band in G-band procedure and its time-or-replication, i.e., both light and dark G-bands were replicated during early, middle, and late S phase. However, it appears that all of the DNA within a single band is replicated together within one third of the S phase.
中国仓鼠成纤维细胞被同步化处理，并给予5-溴脱氧尿苷用于DNA合成，但在S期的一个小时内，培养基中存在胸苷时除外。在下一次有丝分裂中，用33258 Hoechst染色的染色体在通过荧光显微镜拍摄时呈现出带纹。明亮区域对应于S期胸苷暴露期间复制的染色体片段。在任何一小时内一起复制的片段产生了三种不同的模式，分别是早期、中期和晚期S期的特征。大多数荧光区域在大小和位置上与这些染色体的G带相对应。在G带染色过程中，一条带的染色行为与其复制时间没有相关性，即亮G带和暗G带在S期的早期、中期和晚期都会复制。然而，似乎单个带内的所有DNA在S期的三分之一时间内一起复制。
