Department of Biological Science, Florida State University, Tallahassee, Florida 32306, USA.
Cold Spring Harb Perspect Biol. 2021 Jul 1;13(7):a040162. doi: 10.1101/cshperspect.a040162.
Immediately following the discovery of the structure of DNA and the semi-conservative replication of the parental DNA sequence into two new DNA strands, it became apparent that DNA replication is organized in a temporal and spatial fashion during the S phase of the cell cycle, correlated with the large-scale organization of chromatin in the nucleus. After many decades of limited progress, technological advances in genomics, genome engineering, and imaging have finally positioned the field to tackle mechanisms underpinning the temporal and spatial regulation of DNA replication and the causal relationships between DNA replication and other features of large-scale chromosome structure and function. In this review, we discuss these major recent discoveries as well as expectations for the coming decade.
在发现 DNA 结构和双亲 DNA 序列以半保留方式复制成两条新的 DNA 链之后，很明显，DNA 复制是在细胞周期的 S 期以时间和空间的方式组织的，与核内染色质的大规模组织相关。经过几十年的有限进展，基因组学、基因组工程和成像技术的进步终于使该领域能够解决 DNA 复制的时间和空间调控的机制以及 DNA 复制与大规模染色体结构和功能的其他特征之间的因果关系。在这篇综述中，我们讨论了这些最近的重大发现以及对未来十年的期望。
