Hagopian H K, Ingram V M
J Cell Biol. 1971 Nov;51(21):440-51. doi: 10.1083/jcb.51.2.440.
The erythropoietic area of very early chick embryos was cultured as a tissue for up to nine days to study the changes in red cell type and hemoglobin type, the cell cycle time, the cell population kinetics, and the DNA synthetase activity of these cells. It was found that the area vasculosa without the participation of the embryo proper contained the information and the timing mechanism required to produce not only the early primitive erythroid cell population, but also in due course, the later definitive cell type, each with its appropriate hemoglobin types. Also the precursors of the definitive cell type are active in DNA synthesis and therefore are probably in cycle very early in the culture period.
将非常早期鸡胚的造血区域作为组织培养长达九天，以研究红细胞类型和血红蛋白类型的变化、细胞周期时间、细胞群体动力学以及这些细胞的DNA合成酶活性。结果发现，在没有胚胎本身参与的情况下，血管区包含产生早期原始红细胞群体以及随后适时产生后期定型细胞类型所需的信息和时间机制，每种细胞类型都有其相应的血红蛋白类型。此外，定型细胞类型的前体细胞在DNA合成中活跃，因此可能在培养期的早期就处于细胞周期中。
