De la Chapelle A, Fantoni A, Marks P A
Proc Natl Acad Sci U S A. 1969 Jul;63(3):812-9. doi: 10.1073/pnas.63.3.812.
The relationship between the synthesis and replication of DNA and the capacity to synthesize specialized protein on a stable messenger RNA has been examined in yolk sac erythroid cells differentiating in vivo in fetal mice. Evidence is presented that by day 11 of gestation, at least 98 per cent of yolk sac erythroid cells are synthesizing hemoglobin. These cells are shown to incorporate (3)H-thymidine and to replicate, with more than twofold increase in cell number between days 10 and 11. Division of these cells continues through day 13. Hemoglobin formation in these cells is resistant to actinomycin D from day 10. During this period, ribosomal content per cell decreases by more than a factor of 2. These data indicate that in yolk sac erythroid cells, hemoglobin formation proceeds independent of continued RNA formation in cells capable of DNA synthesis and replication.
在胎鼠体内分化的卵黄囊红细胞中，研究了DNA的合成与复制以及在稳定信使RNA上合成特定蛋白质的能力之间的关系。有证据表明，到妊娠第11天，至少98%的卵黄囊红细胞正在合成血红蛋白。这些细胞被证明能掺入³H-胸腺嘧啶并进行复制，在第10天到第11天之间细胞数量增加了两倍多。这些细胞的分裂一直持续到第13天。从第10天起，这些细胞中的血红蛋白形成对放线菌素D具有抗性。在此期间，每个细胞的核糖体含量下降了两倍多。这些数据表明，在卵黄囊红细胞中，血红蛋白的形成在能够进行DNA合成和复制的细胞中独立于持续的RNA形成而进行。
