钩端螺旋体凝集吸附试验的标准化建议。
Proposed standardization of the agglutination-adsorption test for Leptospira.
作者信息
Babudieri B
出版信息
Bull World Health Organ. 1971;44(6):795-810.
The systematics of the Leptospira is based on the agglutination test and on the cross-agglutination-adsorption test. To obtain reproducible and comparable results these tests must be carried out by standard techniques, but the accuracy of the tests is in any case limited since immune sera prepared from animals do not always react uniformly. Moreover, the micro-organisms used in the tests have specific requirements and do not always develop properly in synthetic media. The various factors that influence the results of agglutination-adsorption tests were investigated and evaluated. Taking the results of these studies into account, a standardized method for agglutination-adsorption tests is proposed and described.
钩端螺旋体的分类学是基于凝集试验和交叉凝集吸附试验。为了获得可重复和可比的结果，这些试验必须采用标准技术进行，但无论如何，试验的准确性是有限的，因为从动物制备的免疫血清并非总是表现出一致的反应。此外，试验中使用的微生物有特定要求，在合成培养基中并不总是能正常生长。对影响凝集吸附试验结果的各种因素进行了研究和评估。考虑到这些研究结果，提出并描述了一种凝集吸附试验的标准化方法。
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