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[在兔体生命活动停止后及复苏过程中标记氨基酸掺入兔器官和组织蛋白质的情况]

[Incorporation of labeled amino acids into proteins of organs and tissues of rabbit after cessation of vital activity of the body and during resuscitation].

作者信息

Konikova A S, Pogosova A V, Nikulin V I

出版信息

Vopr Med Khim. 1971 Jul-Aug;17(4):369-73.

PMID:5317617
Abstract

Protein synthesis was assayed through incorporation of labelled amino acids (I-C14-glycine and S15-methionine) into proteins of organs and tissues of rabbit after cessation of life activity of the organism and during reanimation. It is established that after death, protein synthesis ceased in different organs and tissues not at the same time. First of all, protein synthesis stops in the spleen while in the pancreas this process persists for a longest time. Interruption of assimilatory processes after cessation of life is reversible, but restoration of the process of protein synthesis in various organs occurs not uniformly. Ambiguous restoration of precursor utilisation by organs in the process of protein biosynthesis reflects characteristics of the course of this assimilatory process in resuscitated+ animals at early stages after reanimation.

摘要

通过将标记氨基酸(I-C14-甘氨酸和S15-甲硫氨酸)掺入兔子机体生命活动停止后及复苏过程中各器官和组织的蛋白质中,来测定蛋白质合成。已确定,死后不同器官和组织中的蛋白质合成并非同时停止。首先,脾脏中的蛋白质合成停止,而胰腺中的这一过程持续时间最长。生命停止后同化过程的中断是可逆的,但不同器官中蛋白质合成过程的恢复并不一致。在复苏动物复苏后早期,蛋白质生物合成过程中各器官对前体利用的恢复情况不明确,这反映了该同化过程的进程特点。

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