Fagraeus A, Espmark J A, Jonsson J
Immunology. 1965 Aug;9(2):161-75.
Mixed haemadsorption should be regarded as an application of the mixed antiglobulin reaction to situations where the antigen is sessile on a glass surface. Antibody attached to the antigen when exposing the latter to an antiserum is traced by red cells carrying an antiglobulin layer which makes them adsorb to the antibody. The indicator cells are prepared by coating them first with a layer of γ-globulin from the animal species, the antibody globulin of which they are intended to trace, and then with a layer of the corresponding antiglobulin. The most effective indicator cells were obtained by attaching antibody to natural receptors on the red cells to achieve their first coating of γ-globulin. The preparation of indicator cells for tracing antibodies from a number of species, including human, is described. The mixed haemadsorption technique is highly specific and has a sensitivity which is comparable to that of the most sensitive serological techniques. Test procedures adapted for different purposes are outlined and a number of applications to experimental and clinical problems are reviewed.
混合血细胞吸附应被视为混合抗球蛋白反应在抗原固定于玻璃表面情况下的一种应用。当将抗原暴露于抗血清时，附着于抗原的抗体可通过带有抗球蛋白层的红细胞来追踪，该抗球蛋白层使红细胞吸附到抗体上。指示细胞的制备方法是，首先用来自特定动物物种的一层γ球蛋白包被细胞，而该动物物种的抗体球蛋白正是它们要追踪的对象，然后再用一层相应的抗球蛋白包被。通过将抗体附着于红细胞上的天然受体以实现其首次γ球蛋白包被，可获得最有效的指示细胞。本文描述了用于追踪包括人类在内多种物种抗体的指示细胞的制备方法。混合血细胞吸附技术具有高度特异性，其灵敏度与最灵敏的血清学技术相当。概述了适用于不同目的的检测程序，并综述了该技术在实验和临床问题中的一些应用。
