TOENDER O, MILGROM F, WITEBSKY E
J Exp Med. 1964 Feb 1;119(2):265-74. doi: 10.1084/jem.119.2.265.
The mixed agglutination procedure was applied to tissue sections. Microtome sections of bovine tissue were placed on coverglasses, fixed by acetone or formalin, and incubated with various dilutions of rabbit antisera. The binding of antibodies to the tissue sections was detected by the addition of an indicator system composed of sheep erythrocytes sensitized by subagglutinating doses of the corresponding rabbit antiserum, and agglutinated by goat antiserum to rabbit serum. In positive reactions the indicator cells covered the tissue, whereas in negative tests, the erythrocytes detached and the tissue appeared uncovered. It was demonstrated that the method is capable of detecting both saline-extractable and saline-non-extractable antigens. In addition to species-specific antigens, organ-specific antigens of adrenal and brain were detected. The method was characterized by a very high sensitivity in detecting antibodies. Its possible application as a tool for investigations on tissue antigens and antibodies was discussed.
混合凝集试验应用于组织切片。将牛组织的切片置于盖玻片上，用丙酮或福尔马林固定，然后与不同稀释度的兔抗血清孵育。通过加入由亚凝集剂量的相应兔抗血清致敏、并能被山羊抗兔血清凝集的绵羊红细胞组成的指示系统，来检测抗体与组织切片的结合。在阳性反应中，指示细胞覆盖组织，而在阴性试验中，红细胞分离，组织看起来未被覆盖。结果表明该方法能够检测盐水可提取和盐水不可提取的抗原。除了种属特异性抗原外，还检测到了肾上腺和脑的器官特异性抗原。该方法在检测抗体方面具有非常高的灵敏度。讨论了其作为研究组织抗原和抗体工具的可能应用。
