Osoba D
J Exp Med. 1965 Sep 1;122(3):633-50. doi: 10.1084/jem.122.3.633.
When neonatally thymectomized CBA mice were implanted at 9 to 12 days of age with Millipore diffusion chambers (pore size, 0.1 micro) containing either syngeneic or allogeneic neonatal thymus, they were subsequently found to have the capacity to reject skin homografts and to form antibodies to sheep erythrocytes. In spite of displaying restored immune reactivity, thymectomized mice bearing thymus-filled diffusion chambers still had a lymphopenia and diminished numbers of small lymphocytes in their spleens, lymph nodes and Peyer's patches. Comparison of the lymphoid organs of these mice with those of the thymectomized control mice did not reveal any appreciable difference in the numbers of primary follicles or small lymphocytes. It is postulated that the thymus humoral factor induced immunological competence in lymphoid cells which had left the thymus prior to neonatal thymectomy. The paucity of circulating and tissue small lymphocytes in thymectomized animals, the immune reactivity of which was restored by thymus tissue in diffusion chambers, argues against the theory that the thymus humoral factor has a lymphocytosis-stimulating effect. There was no restoration of immune reactivity in those neonatally thymectomized mice which had been implanted with diffusion chambers containing neonatal or adult spleens, or adult lymph nodes. Thus, the competence-inducing factor is elaborated by the thymus but not by the spleen or lymph nodes. Allogeneic (C57Bl) neonatal thymus tissue, enclosed within diffusion chambers, had the capacity to restore the immune reactivity of totally thymectomized CBA mice, not only to skin homografts of a totally unrelated strain (Ak), but also to grafts isogeneic with the donor of the allogeneic thymus. Therefore, there is no strain barrier to the action of thymus humoral factor. To explain the apparent lack of full participation of thymus lymphocytes in immune reactions it is postulated that thymus lymphocytes are functionally immature in situ, and that they leave the thymus before attaining immunological competence. In the periphery, they undergo further maturation under the influence of the competence-inducing factor produced by the thymus.
当新生期切除胸腺的CBA小鼠在9至12日龄时植入含有同基因或异基因新生胸腺的微孔扩散小室（孔径0.1微米）后，随后发现它们有能力排斥皮肤同种异体移植物并形成抗绵羊红细胞抗体。尽管表现出恢复的免疫反应性，但携带充满胸腺的扩散小室的切除胸腺小鼠仍有淋巴细胞减少，其脾脏、淋巴结和派伊尔结中的小淋巴细胞数量减少。将这些小鼠的淋巴器官与切除胸腺的对照小鼠的淋巴器官进行比较，未发现初级滤泡或小淋巴细胞数量有任何明显差异。据推测，胸腺体液因子在新生期胸腺切除术前就已离开胸腺的淋巴细胞中诱导免疫能力。切除胸腺的动物循环和组织中小淋巴细胞数量稀少，其免疫反应性通过扩散小室中的胸腺组织得以恢复，这与胸腺体液因子具有刺激淋巴细胞增多作用的理论相悖。那些植入含有新生或成年脾脏或成年淋巴结的扩散小室的新生期切除胸腺的小鼠，其免疫反应性并未恢复。因此，诱导能力的因子是由胸腺而非脾脏或淋巴结产生的。包裹在扩散小室中的异基因（C57Bl）新生胸腺组织不仅有能力恢复完全切除胸腺的CBA小鼠对完全不相关品系（Ak）的皮肤同种异体移植物的免疫反应性，而且还能恢复对与异基因胸腺供体同基因的移植物的免疫反应性。因此，胸腺体液因子的作用不存在品系障碍。为了解释胸腺淋巴细胞明显未充分参与免疫反应的现象，据推测胸腺淋巴细胞在原位功能不成熟，并且它们在获得免疫能力之前就离开胸腺。在周围组织中，它们在胸腺产生的诱导能力因子的影响下进一步成熟。
