Kane R E, Forer A
J Cell Biol. 1965 Jun;25(3):Suppl:31-9. doi: 10.1083/jcb.25.3.31.
The fibrous structure of the mitotic apparatus (MA) isolated from dividing sea urchin eggs undergoes no changes visible in phase contrast during extended storage, but the solubility of the MA rapidly decreases after isolation. Polarization microscopy shows that a decrease in the birefringence of the MA also occurs after isolation and is correlated with the loss of solubility. This loss of birefringence indicates that some structural change takes place during this period, and such a change was demonstrated by means of electron microscopy. The tubular filaments which form the spindle of the intracellular MA and of the freshly isolated MA were found to break down during storage to rows of dense granules, this loss of continuity presumably accounting for the loss of birefringence. The interrelations of the observed changes and the significance of these observations for investigations on the isolated MA are discussed.
从分裂的海胆卵中分离出的有丝分裂器(MA)的纤维结构,在长时间储存期间,在相差显微镜下未观察到可见变化,但MA的溶解度在分离后迅速降低。偏振显微镜显示,MA的双折射在分离后也会降低,并且与溶解度的丧失相关。双折射的丧失表明在此期间发生了一些结构变化,并且通过电子显微镜证实了这种变化。发现构成细胞内MA和新分离的MA纺锤体的管状细丝在储存期间分解成一排排致密颗粒,这种连续性的丧失大概是双折射丧失的原因。讨论了观察到的变化之间的相互关系以及这些观察结果对分离的MA研究中的意义。