Guski H, Heidelberger H, Osswald U
Z Exp Chir. 1979;12(3):146-53.
The authors describe device and functioning of a new efficacious membrane thin-layer oxygenator, specially developed for experiments on the isolated hearts of small animals. Compared with customary oxygenators with direct oxygenation, advantages arise from the separation of gas and perfusion solution, and its small volume for filling (7,5 ml). The diffusion capacity of the oxygenator (exchange surface 980 cm2) was tried by means of a hemoglobin-free modified Krebs-Henseleit solution with various flow values at 37 degrees C, depending on the gage of the layer. At the optimal gage of 0,15 mm and a flow value of 15 ml/minute a perfusion solution can be oxygenated from 0 to 570 torr, and an airequilibrated solution up to 646 torr. Measurements of oxygen consumption in the isolated perfused rat heart proved that the diffusion capacity of the membrane thin-layer oxygenator will suffice the oxygenation of the coronary minute volume of the arrested heart according to Langendorff as well as of the working heart according to Morgan. Primarily this oxygenator is useful for experimental organ perfusion in metabolic and functional tests, and organ preservation. Its functioning principles, however, also permit its use as thins-layer dialysator.
作者描述了一种新型高效膜式薄层氧合器的装置和功能，该氧合器是专门为小动物离体心脏实验开发的。与传统的直接氧合氧合器相比，气体和灌注液的分离以及其较小的填充体积（7.5毫升）具有优势。在37摄氏度下，使用不含血红蛋白的改良克雷布斯 - 亨泽莱特溶液，通过不同的流量值，根据层的厚度，对氧合器（交换面积980平方厘米）的扩散能力进行了测试。在最佳厚度0.15毫米和流量值15毫升/分钟时，灌注液可从0氧合到570托，空气平衡溶液可氧合到646托。对离体灌注大鼠心脏的氧消耗测量证明，膜式薄层氧合器的扩散能力足以满足按照兰根多夫法对停搏心脏以及按照摩根法对工作心脏的冠状动脉分钟流量的氧合需求。这种氧合器主要用于代谢和功能测试中的实验性器官灌注以及器官保存。然而，其工作原理也允许将其用作薄层透析器。
