Alexander B, Von Arnem T, Aslam M, Kolhe P S, Benjamin I S
Lab Invest. 1984 May;50(5):597-603.
Silastic membrane tubing oxygenators possess valuable atraumatic properties, but no satisfactory miniature system for isolated organ perfusion has been achieved. We describe the construction of a new type of miniaturized Silastic membrane tubing oxygenator, 1.5 m long, 1.5-mm diameter, 75-micron wall thickness, incorporated in a single self-supporting unit readily applicable to isolated organ perfusion because of its simplicity, durability, and small prime volume of less than 3 ml. In vitro experiments using out-dated human banked blood demonstrated that the unit can effectively oxygenate blood of hematocrit 35% at flow rates of at least 10 ml/minute and that higher flow rates may be used to oxygenate blood or perfusates of a lower hematocrit. The results were verified in three different practical experimental applications: isolated rat liver, rabbit heart, and rat free skin flap perfusion.
硅橡胶膜管氧合器具有宝贵的无创伤特性，但尚未实现用于离体器官灌注的令人满意的微型系统。我们描述了一种新型的小型化硅橡胶膜管氧合器的构造，其长1.5米，直径1.5毫米，壁厚75微米，包含在一个单一的自支撑单元中，因其简单、耐用且预充量小于3毫升而易于应用于离体器官灌注。使用过期的人类库存血液进行的体外实验表明，该装置能够以至少10毫升/分钟的流速有效地为血细胞比容为35%的血液供氧，并且可以使用更高的流速为血细胞比容较低的血液或灌注液供氧。这些结果在三种不同的实际实验应用中得到了验证：大鼠离体肝脏、兔心脏和大鼠游离皮瓣灌注。
