痢疾志贺氏菌及大肠杆菌-痢疾志贺氏菌杂交体中β-D-半乳糖苷酶的性质与调控
Properties and regulation of the beta-D-galactosidase in Shigella dysenteriae and in Escherichia coli-Shigella dysenteriae hybrids.
作者信息
Sarkar S
出版信息
J Bacteriol. 1966 Apr;91(4):1477-88. doi: 10.1128/jb.91.4.1477-1488.1966.
Abstract
Sarkar, S. (Massachusetts Institute of Technology, Cambridge). Properties and regulation of the beta-d-galactosidase in Shigella dysenteriae and in Escherichia coli-Shigella dysenteriae hybrids. J. Bacteriol. 91:1477-1488. 1966.-Shigella dysenteriae strain 60 has a beta-d-galactosidase related to that of Escherichia coli but more heat-sensitive and with a turnover number about 10 times lower. Hybridization by transduction produces strains with enzymes of intermediate properties by recombination within the z gene. Both E. coli and S. dysenteriae have a regulatory i(+) gene. Recombination between i(-) mutants of the two organisms leads to restoration of the i(+) genotype. In S. dysenteriae 60, most of the i(-) mutants are subject to genetic suppression by suppressor mutations at unlinked loci. The effect of these suppressors on the products of the suppressed i(-) genes is discussed.
摘要
萨卡尔，S.（麻省理工学院，剑桥）。痢疾志贺氏菌及大肠杆菌 - 痢疾志贺氏菌杂交体中β - d - 半乳糖苷酶的性质与调控。《细菌学杂志》91:1477 - 1488。1966年。 - 痢疾志贺氏菌60株具有一种与大肠杆菌的β - d - 半乳糖苷酶相关的酶，但对热更敏感，周转数约低10倍。通过转导杂交产生的菌株在z基因内通过重组具有中间性质的酶。大肠杆菌和痢疾志贺氏菌都有一个调控性的i(+)基因。两种生物体的i(-)突变体之间的重组导致i(+)基因型的恢复。在痢疾志贺氏菌60中，大多数i(-)突变体受到非连锁位点上抑制突变的遗传抑制。讨论了这些抑制子对被抑制的i(-)基因产物的影响。
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