Markovitz A, Rosenbaum N, Baker B
J Bacteriol. 1968 Jul;96(1):221-6. doi: 10.1128/jb.96.1.221-226.1968.
When Shigella dysenteriae strain 60 is used as a donor and Escherichia coli K-12 strains that are ultraviolet (UV)-sensitive, mucoid, and proline-requiring (Pro(-)) are employed as recipients, selection for Pro(+) yields 2 to 6% nonmucoid clones. All of the nonmucoid clones examined are UV-resistant. Most of the nonmucoid UV-resistant transductants are partial diploids for the genes being studied. When these Shigella-Escherichia hybrids are used as donors with the same E. coli recipients, the cotransduction of Pro(+) and nonmucoidness is greatly increased (59 to 94% cotransduction). All of these nonmucoid transductants examined were also UV-resistant. The results indicate that Shigella contains an allele (designated ShproC(+)) homologous to proC of E. coli and a second linked allele (designated ShcapR(+)) homologous to the capR allele of E. coli. The ShcapR(+) allele changes the phenotype of certain E. coli strains from mucoid UV-sensitive (capR6) or very sensitive (capR9) to nonmucoid and UV-resistant. Unanticipated capR allele interactions in the partial diploid hybrids are described.
当痢疾志贺氏菌60菌株用作供体，而将对紫外线（UV）敏感、黏液状且需要脯氨酸（Pro(-)）的大肠杆菌K-12菌株用作受体时，选择Pro(+)会产生2%至6%的非黏液状克隆。所有检测的非黏液状克隆均对紫外线具有抗性。大多数非黏液状的抗紫外线转导子在所研究的基因方面是部分二倍体。当这些志贺氏菌 - 大肠杆菌杂种用作供体与相同的大肠杆菌受体进行杂交时，Pro(+)和非黏液状的共转导显著增加（共转导率为59%至94%）。所有检测的这些非黏液状转导子也都对紫外线具有抗性。结果表明，志贺氏菌含有一个与大肠杆菌proC同源的等位基因（命名为ShproC(+)）以及另一个与大肠杆菌capR等位基因同源的连锁等位基因（命名为ShcapR(+)）。ShcapR(+)等位基因可将某些大肠杆菌菌株的表型从黏液状对紫外线敏感（capR6）或非常敏感（capR9）转变为非黏液状且对紫外线具有抗性。文中还描述了部分二倍体杂种中意外的capR等位基因相互作用。
