Jakubowska-Naziembło B
Acta Physiol Pol. 1979 Sep-Dec;30(5-6):597-606.
The purpose of this work was to compare the oxytocic activity of plasma and posterior pituitary lobe extract in rats after sham operation, ovariectomy and after subcutaneous implantation of stilboestrol or progesterone tablets in ovariectomized rats. On the 5th day after ovariectomy or implantation of hormones, sample of 2 ml of blood were obtained under urethane anaesthesia from the cephalic end of the right external jugular vein, and the animals were killed by decapitation. The posterior pituitary lobe was removed and homogenized in 0.9% NaCl solution acidified with glacial acetic acid. The oxytocic activity of plasma and extracts of the posterior pituitary lobe was determined by the method of Van Dongen and Hays on fragments of lactating rat mammary tissue. On the 5th day after ovariectomy or implantation of stilboestrol the oxytocic activity was found to be significantly increased in the plasma and posterior pituitary lobe, and after progesterone implantation it was decreased in the posterior pituitary lobe.
本研究旨在比较假手术、卵巢切除术后以及在去卵巢大鼠皮下植入己烯雌酚或孕酮片后，大鼠血浆和垂体后叶提取物的催产活性。在卵巢切除或激素植入后的第5天，在氨基甲酸乙酯麻醉下，从右颈外静脉头端采集2 ml血液样本，然后断头处死动物。取出垂体后叶，在含冰醋酸酸化的0.9%氯化钠溶液中匀浆。采用范东根和海斯的方法，以泌乳大鼠乳腺组织片段测定血浆和垂体后叶提取物的催产活性。在卵巢切除或植入己烯雌酚后的第5天，发现血浆和垂体后叶的催产活性显著增加，而在植入孕酮后，垂体后叶的催产活性降低。
