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福氏气单胞菌β-半乳糖苷酶的纯化及特性分析

Purification and characterization of the beta-galactosidase of Aeromonas formicans.

作者信息

Rohlfing S R, Crawford I P

出版信息

J Bacteriol. 1966 Mar;91(3):1085-97. doi: 10.1128/jb.91.3.1085-1097.1966.

DOI:10.1128/jb.91.3.1085-1097.1966
PMID:5326700
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC316000/
Abstract

Rohlfing, S. R. (Western Reserve University, Cleveland, Ohio), and I. P. Crawford. Purification and characterization of the beta-galactosidase of Aeromonas formicans. J. Bacteriol. 91:1085-1097. 1966.-The beta-galactosidase of Aeromonas formicans was purified by diethylaminoethyl cellulose chromatography and gel filtration on Sephadex G-200. The properties of the enzyme molecule were compared with purified beta-galactosidase from Escherichia coli. The sedimentation coefficients and electrophoretic mobilities of the two enzymes were not significantly different; the electrophoretic mobility of urea-produced subunits of the two enzymes was also similar. The stabilities of the two enzymes to denaturing agents provided measurable differences; E. coli beta-galactosidase is relatively more heat-stable and more resistant to the action of urea. The amino acid compositions of the two proteins revealed significant differences in several amino acids, particularly alanine, arginine, glycine, and leucine. The comparisons cited suggest that A. formicans and E. coli are not completely unrelated, for their beta-galactosidases show considerable structural similarity.

摘要

罗尔芬，S. R.（俄亥俄州克利夫兰市西储大学），以及I. P. 克劳福德。蚁气单胞菌β-半乳糖苷酶的纯化与特性。《细菌学杂志》91:1085 - 1097。1966年。——蚁气单胞菌的β-半乳糖苷酶通过二乙氨基乙基纤维素色谱法和葡聚糖凝胶G - 200凝胶过滤法进行纯化。将该酶分子的特性与来自大肠杆菌的纯化β-半乳糖苷酶进行了比较。两种酶的沉降系数和电泳迁移率没有显著差异；两种酶经尿素处理产生的亚基的电泳迁移率也相似。两种酶对变性剂的稳定性存在可测量的差异；大肠杆菌β-半乳糖苷酶相对更耐热且更耐尿素的作用。两种蛋白质的氨基酸组成在几种氨基酸上存在显著差异，特别是丙氨酸、精氨酸、甘氨酸和亮氨酸。所引用的比较表明，蚁气单胞菌和大肠杆菌并非完全没有关联，因为它们的β-半乳糖苷酶显示出相当大的结构相似性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa85/316000/abd4886fa602/jbacter00420-0225-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa85/316000/ffe28f8dd752/jbacter00420-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa85/316000/c869e617eee3/jbacter00420-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa85/316000/18ec66de942a/jbacter00420-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa85/316000/41372e0f66d0/jbacter00420-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa85/316000/b394104ccf22/jbacter00420-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa85/316000/abd4886fa602/jbacter00420-0225-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa85/316000/ffe28f8dd752/jbacter00420-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa85/316000/c869e617eee3/jbacter00420-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa85/316000/18ec66de942a/jbacter00420-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa85/316000/41372e0f66d0/jbacter00420-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa85/316000/b394104ccf22/jbacter00420-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa85/316000/abd4886fa602/jbacter00420-0225-a.jpg

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