Effect of prostaglandin E2 and 15(R)15 methyl prostaglandin E2 on canine gastric parietal cell: an ultrastructural study.

The effect of prostaglandin E2 (PGE2) and 15(R)15 methyl prostaglindin E2 (15(R)15 PGE2) on the gastric parietal cell was studied in seven and eight dogs, respectively. Another four dogs were used as controls. Gastric biopsies were examined by the electron microscope and the secretory activity of the parietal cells was assessed by morphometrically estimating the proportion of the parietal cell cytoplasm occupied by tubulovesicles as well as the ratio of extramitochondrial cytoplasm:mitochondria. Both 15(R)15 PGE2 and PGE2 increased the latter one hour after administration but only 15(R)15 PGE2 increased the proportion of tubulovesicles. The data suggest that secretory activity of parietal cells is impaired, especially after treatment with 15(R)15 GE2.