Soll A H
J Clin Invest. 1980 May;65(5):1222-9. doi: 10.1172/JCI109777.
The effects of prostaglandins E2 and I2 on accumulation of [14C]aminopyrine and the generation of cyclic AMP by fractions of dispersed canine gastric mucosal cells, enriched in their content of parietal cells, have been studied. The parietal cell content of the fractions was enriched to between 43 and 70% using an elutriator rotor. The accumulation of [14C]aminopyrine was used as the index of parietal cell response to stimulation. Prostaglandin E2 (PGE2, 0.1 nM-0.1 mM) inhibited histamine stimulated aminopyrine uptake but did not block the response to carbachol, gastrin, or dibuturyl cyclic AMP. PGE2 did, however, inhibit aminopyrine uptake stimulated by carbachol and gastrin when the response to these agents was potentiated by histamine. PGE2 (0.1 NM-0.1 mM) inhibited histamine-stimulated cyclic AMP production in a dose-dependent fashion with maximal inhibition at 1 microM PGE2. Prostacyclin also inhibited both histamine-stimulated aminopyrine accumulation and histamine-stimulated cyclic AMP production. In the absence of added histamine, PGE2 in concentrations above 1 microM and prostacyclin in concentrations above 10 microM stimulated cyclic AMP production, probably by acting on the nonparietal cells as shown in previous studies. These present data are consistent with the hypothesis that prostaglandins E2 and I2 inhibit the response of isolated parietal cells to histamine by specifically blocking histamine-stimulated cyclic AMP production.
研究了前列腺素E2和I2对富含壁细胞的犬胃黏膜分散细胞组分中[14C]氨基比林积累及环磷酸腺苷生成的影响。使用淘析转子将各组分的壁细胞含量富集至43%至70%。[14C]氨基比林的积累用作壁细胞对刺激反应的指标。前列腺素E2（PGE2，0.1 nM - 0.1 mM）抑制组胺刺激的氨基比林摄取，但不阻断对卡巴胆碱、胃泌素或二丁酰环磷酸腺苷的反应。然而，当组胺增强对这些药物的反应时，PGE2确实抑制了卡巴胆碱和胃泌素刺激的氨基比林摄取。PGE2（0.1 nM - 0.1 mM）以剂量依赖性方式抑制组胺刺激的环磷酸腺苷生成，在1 μM PGE2时抑制作用最大。前列环素也抑制组胺刺激的氨基比林积累和组胺刺激的环磷酸腺苷生成。在没有添加组胺的情况下，浓度高于1 μM的PGE2和浓度高于10 μM的前列环素刺激环磷酸腺苷生成，可能如先前研究所示，是通过作用于非壁细胞。这些现有数据与以下假设一致，即前列腺素E2和I2通过特异性阻断组胺刺激的环磷酸腺苷生成来抑制分离的壁细胞对组胺的反应。
