Johnston C K, Lester G H
Ann Clin Biochem. 1979 Jul;16(4):213-6. doi: 10.1177/000456327901600150.
We describe a simple, rapid procedure for the estimation of carbamazepine in plasma. Protein is precipitated, and extraction is achieved by the addition of acetonitrile containing the internal standard N-acetyltryptophan ethyl ester. Separation is by reverse-phase high-pressure liquid chromatography with an acetonitrile: water mobile phase, and detection is by UV absorption at 280 nm. Total retention time is less than 7 minutes. Initial results gave within-batch and between-batch coefficients of variation of less than 2%, and mean recovery of 97%. The method is free from interference by other common anticonvulsant drugs.
我们描述了一种简单、快速的血浆中卡马西平的估算方法。蛋白质沉淀后，通过加入含有内标N - 乙酰色氨酸乙酯的乙腈进行萃取。采用乙腈 - 水流动相的反相高压液相色谱进行分离，通过在280nm处的紫外吸收进行检测。总保留时间小于7分钟。初步结果显示批内和批间变异系数均小于2%，平均回收率为97%。该方法不受其他常见抗惊厥药物的干扰。
