Elyas A A, Ratnaraj N, Goldberg V D, Lascelles P T
J Chromatogr. 1982 Aug 13;231(1):93-101. doi: 10.1016/s0378-4347(00)80512-5.
Carbamazepine and carbamazepine-10,11-epoxide were separated by high-performance liquid chromatography (HPLC) with acetonitrile-water as mobile phase, and detection was effected by UV absorption at 215 nm with a total retention time of less than 10 min. Plasma samples were extracted with dichloromethane and 4 M sodium hydroxide, and 10-methoxy-carbamazepine was added as internal standard. Other commonly used anticonvulsant drugs present in plasma showed no significant interference. The within-batch coefficient of variation for carbamazepine was 4.9% and carbamazepine-10,11-epoxide 5.9%. Between-batch coefficients of variation were 3.7% and 5.3%, respectively. Mean recovery for carbamazepine was 100.2% and for carbamazepine-10,11-epoxide 100.6%. This HPLC method was compared with both an enzyme immunoassay procedure (EMIT) and a gas-liquid chromatographic (GLC) method. Correlation coefficient between HPLC/EMIT for carbamazepine was 0.983, HPLC/GLC carbamazepine 0.988 and HPLC/GLC carbamazepine-10,11-epoxide 0.981.
采用以乙腈 - 水为流动相的高效液相色谱法（HPLC）分离卡马西平和卡马西平 - 10,11 - 环氧化物，通过在215 nm处的紫外吸收进行检测，总保留时间小于10分钟。血浆样品用二氯甲烷和4 M氢氧化钠萃取，并加入10 - 甲氧基 - 卡马西平作为内标。血浆中存在的其他常用抗惊厥药物无明显干扰。卡马西平的批内变异系数为4.9%，卡马西平 - 10,11 - 环氧化物为5.9%。批间变异系数分别为3.7%和5.3%。卡马西平的平均回收率为100.2%，卡马西平 - 10,11 - 环氧化物为100.6%。将该HPLC方法与酶免疫分析方法（EMIT）和气液色谱法（GLC）进行了比较。卡马西平的HPLC/EMIT之间的相关系数为0.983，卡马西平的HPLC/GLC为0.988，卡马西平 - 10,11 - 环氧化物的HPLC/GLC为0.981。
