Somerville R L
Science. 1966 Dec 23;154(3756):1585-7. doi: 10.1126/science.154.3756.1585.
Hybrids hemizygous for the tryptophan genes were prepared by episomal transfer of an Escherichia coli element into Salmonella typhimurium. Regulation of enzyme production by hybrids carrying wild-type E. coli genes in response to changes in the growth medium occurs in precisely the same manner as in haploid E. coli wild type. Mutant alleles of the anthranilate synhetase gene of E. coli which prevent derepression in E. coli function identically in S. typhimurium. At least one Salmonella tryptophan regulatory gene unlinked to the structural genes is known. Any dijferences which may exist between the tryptophan regulatory genes of E. coli and Salmonella have little effect on the regulation of enzyme formation in hybrids.
通过将大肠杆菌元件附加体转移至鼠伤寒沙门氏菌中，制备了色氨酸基因半合子的杂种。携带野生型大肠杆菌基因的杂种对生长培养基变化的酶产生调节，其发生方式与单倍体大肠杆菌野生型完全相同。在大肠杆菌中阻止去阻遏的邻氨基苯甲酸合酶基因突变等位基因，在鼠伤寒沙门氏菌中功能相同。已知至少有一个与结构基因不连锁的沙门氏菌色氨酸调节基因。大肠杆菌和沙门氏菌的色氨酸调节基因之间可能存在的任何差异，对杂种中酶形成的调节影响很小。
