Arrau J, Roblero L
Arch Anat Microsc Morphol Exp. 1979;68(2):73-84.
Gonads of 15 day-old hamster fetuses were grafted under the kidney capsule of adult ovariectomized females. In 81.8% of the grafts, the germ cells developed and completed meiotic prophase; they reached the diplotene stage and gave rise to primordial follicles. These grafts could survive well in the host for at least 20 days. Meiotic prophase was not initiated within 24 h of grafting in contrast to the in vivo condition where it is observed 24 h after birth. In grafted ovaries, 69% of the germ cells were at the leptotene stage on day 3. By day 5, most of them were either at the zygotene (15%) or pachytene stages (60%). Oocytes at the diplotene stage were found from day 5 onwards and on day 7, 30% of the germ cells had already reached this stage. The highest numbers of atretic germ cells could be found on days 1, 9 and 10 post graft. It is noteworthy that the number of germinal cells remaining in the ovary 10 and 20 days after grafting were 11.4% and 12.9% of the total number of germinal cells present in normal animals of the same ages post-partum. This point is discussed in detail.
将15日龄仓鼠胎儿的性腺移植到成年去卵巢雌性动物的肾被膜下。在81.8%的移植性腺中，生殖细胞发育并完成减数分裂前期；它们达到双线期并形成原始卵泡。这些移植性腺在宿主中至少能存活20天。与出生后24小时就观察到减数分裂前期的体内情况不同，移植后24小时内减数分裂前期并未启动。在移植后的卵巢中，第3天69%的生殖细胞处于细线期。到第5天，大多数生殖细胞处于偶线期（15%）或粗线期（60%）。从第5天开始可发现处于双线期的卵母细胞，到第7天，30%的生殖细胞已达到此阶段。移植后第1天、第9天和第10天可发现数量最多的闭锁生殖细胞。值得注意的是，移植后10天和20天卵巢中剩余的生殖细胞数量分别为同年龄产后正常动物生殖细胞总数的11.4%和12.9%。这一点将进行详细讨论。
