Asaba H, Bergström J, Fürst P, Gunnarsson B, Neuhauser M, Oulès R, Yahiel V
Artif Organs. 1979 May;3(2):132-6. doi: 10.1111/j.1525-1594.1979.tb01025.x.
In four regular dialysis patients, the removal of uremic middle molecules was studied during a three-hour hemoperfusion using a column containing 300 gm of activated carbon encapsulated with cellulose. Middle molecules in plasma were determined by high-speed gel filtration followed by gradient elution chromatography. Different adsorption characteristics were shown for the four middle molecule fractions measured. The initial clearances for middle molecule fractions were about 120 ml/min; clearances were 20-50 ml/min after two hours, and less than 25 ml/min, with release of some middle molecule fractions from the column, after three hours. The findings suggest that saturation of the column occurs after two to three hours of perfusion. Thus, prolongation of the perfusion time beyond two hours appears to be of little benefit. The reduction of middle molecules in plasma by a three-hour hemoperfusion was in magnitude, similar to that obtained by three-hour dialysis with a large surface area dialyzer or high-flux membrane.
在4例常规透析患者中，使用装有300克纤维素包裹活性炭的柱子，在3小时血液灌流期间研究了尿毒症中分子的清除情况。血浆中的中分子通过高速凝胶过滤继以梯度洗脱色谱法测定。所测的4种中分子组分表现出不同的吸附特性。中分子组分的初始清除率约为120毫升/分钟；两小时后清除率为20 - 50毫升/分钟，三小时后清除率小于25毫升/分钟，且有一些中分子组分从柱子上释出。这些发现提示，灌注两到三小时后柱子会发生饱和。因此，将灌注时间延长至两小时以上似乎益处不大。三小时血液灌流使血浆中分子减少的幅度，与使用大面积透析器或高通量膜进行三小时透析所获得的幅度相似。
