使用厌氧培养袋从新鲜肉类中分离肉毒梭菌孢子。
Use of the anaerobic pouch in isolating Clostridium botulinum spores from fresh meats.
作者信息
Greenberg R A, Bladel B O, Zingelmann W J
出版信息
Appl Microbiol. 1966 Mar;14(2):223-8. doi: 10.1128/am.14.2.223-228.1966.
Abstract
The anaerobic film pouch was demonstrated to be an effective device for the primary isolation of Clostridium botulinum types A and B spores from raw pork, beef, and chicken. Optimal pasteurization of these meats (for reduction of nonspore microflora without affecting indigenous putrefactive anaerobic spore levels) was 50 min at 60 C. C. botulinum spores were recovered with good precision from meat samples inoculated with mixtures of C. botulinum and Putrefactive Anaerobe 3679 at 1:1 and at 1:99 ratios. Verification of C. botulinum isolates was accomplished by protection testing of subcultures in mice.
摘要
厌氧薄膜袋被证明是从生猪肉、牛肉和鸡肉中初步分离A型和B型肉毒梭菌孢子的有效装置。这些肉类的最佳巴氏杀菌条件（用于减少非芽孢微生物群而不影响本地腐败厌氧芽孢水平）是在60℃下处理50分钟。从接种了肉毒梭菌和腐败厌氧菌3679比例为1:1和1:99混合物的肉类样品中，能以良好的精度回收肉毒梭菌孢子。通过对小鼠亚培养物进行保护试验来完成肉毒梭菌分离株的验证。
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