Quinn R W, Lowry P N
J Bacteriol. 1967 Jun;93(6):1825-31. doi: 10.1128/jb.93.6.1825-1831.1967.
Human tissue cell lines from each of the three primary germinal sources, ectoderm (conjunctiva and carcinoma of the buccal mucosa), entoderm (intestine and liver), and mesoderm (heart and monocytes) were inoculated with group A Streptococcus pyogenes, Staphylococcus aureus, and group D streptococci and were then observed. In addition, the effect of these bacteria on mouse fibroblasts was studied. All of the cell lines appeared to be equally susceptible to damage, but damage to the cells by S. pyogenes occurred only when living, actively multiplying bacteria were in contact with the tissue cells. Streptococcal products in the form of "used" growth medium had no observable effect on the cells. Cytopathogenic effects were first noticed about the time one would expect the bacteria to have reached the end of the log phase of growth. No damage to the tissue cells was noted when group A streptococci were separated from the cells by membrane filter diffusion chambers or dialyzing membranes, but a membrane did not protect cells from deleterious effects of staphylococci or group D streptococci. Group A streptococci survived in the tissue culture medium, but multiplication did not occur unless living tissue cells were present.
从三个主要胚层来源（外胚层（结膜和颊黏膜癌）、内胚层（肠和肝）和中胚层（心脏和单核细胞））获取的人体组织细胞系，接种A组化脓性链球菌、金黄色葡萄球菌和D组链球菌，然后进行观察。此外，还研究了这些细菌对小鼠成纤维细胞的影响。所有细胞系似乎对损伤同样敏感，但只有当活的、正在积极繁殖的细菌与组织细胞接触时，化脓性链球菌才会对细胞造成损伤。“用过的”生长培养基形式的链球菌产物对细胞没有可观察到的影响。细胞病变效应大约在预期细菌达到对数生长期末期时首次被注意到。当通过膜滤扩散室或透析膜将A组链球菌与细胞分离时，未观察到对组织细胞的损伤，但膜不能保护细胞免受葡萄球菌或D组链球菌的有害影响。A组链球菌在组织培养基中存活，但除非有活的组织细胞存在，否则不会繁殖。
