Weibel E R, Kistler G S, Scherle W F
J Cell Biol. 1966 Jul;30(1):23-38. doi: 10.1083/jcb.30.1.23.
Stereological principles provide efficient and reliable tools for the determination of quantitative parameters of tissue structure on sections. Some principles which allow the estimation of volumetric ratios, surface areas, surface-to-volume ratios, thicknesses of tissue or cell sheets, and the number of structures are reviewed and presented in general form; means for their practical application in electron microscopy are outlined. The systematic and statistical errors involved in such measurements are discussed.
体视学原理为确定切片上组织结构的定量参数提供了高效且可靠的工具。本文综述并以一般形式呈现了一些可用于估计体积比、表面积、表面体积比、组织或细胞片层厚度以及结构数量的原理;概述了它们在电子显微镜中的实际应用方法。讨论了此类测量中涉及的系统误差和统计误差。