可溶性核糖核酸对肝脏模板核糖核酸刺激作用的抑制
Inhibition by soluble ribonucleic acid of stimulatory effect of liver template ribonucleic acid.
作者信息
Aaronson S A, Korner A, Munro A J
出版信息
Biochem J. 1966 Nov;101(2):448-53. doi: 10.1042/bj1010448.
Abstract
- Liver soluble RNA (s-RNA) and, to a smaller extent, Escherichia coli s-RNA inhibited the stimulation of [(14)C]leucine incorporation into protein in an E. coli S-30 system brought about by liver microsomal RNA or polyuridylic acid as template. 2. The inhibitory activity was associated with the fraction of the s-RNA possessing transfer-RNA activity. 3. The inhibition was exercised at a stage after charging of the s-RNA with amino acid. 4. Neither the method of preparation of the s-RNA nor its state of amino acid acylation affected its inhibitory action. 5. Stimulation of [(14)C]phenylalanine incorporation by polyuridylic acid or by liver microsomal RNA was not inhibited by addition of s-RNA. 6. It appears that excess of s-RNA inhibits the ambiguous incorporation of leucine with polyuridylic acid and also that something similar occurs with a natural template. 7. Estimation of messenger activity of samples of RNA should be carried out only after removal of s-RNA.
摘要
- 肝脏可溶性核糖核酸（s-RNA）以及程度较小的大肠杆菌s-RNA，抑制了在以肝脏微粒体RNA或多聚尿苷酸为模板的大肠杆菌S-30系统中，[（14）C]亮氨酸掺入蛋白质的刺激作用。2. 抑制活性与具有转移RNA活性的s-RNA部分相关。3. 抑制作用发生在s-RNA用氨基酸负载之后的阶段。4. s-RNA的制备方法及其氨基酸酰化状态均不影响其抑制作用。5. 添加s-RNA不会抑制多聚尿苷酸或肝脏微粒体RNA对[（14）C]苯丙氨酸掺入的刺激作用。6. 似乎过量的s-RNA会抑制亮氨酸与多聚尿苷酸的模糊掺入，并且天然模板也会发生类似情况。7. RNA样品的信使活性估计应仅在去除s-RNA后进行。
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