Sinskey T J, Silverman G J, Goldblith S A
Appl Microbiol. 1967 Jan;15(1):22-30. doi: 10.1128/am.15.1.22-30.1967.
Salmonella typhimurium survived freeze-drying at a platen temperature of 120 F (48.9 C) and also, though to a much lesser degree, at 160 F (82.6 C). The extent of the survival at these temperatures was dependent on the composition of the model system employed. The incidence of damage immediately after freeze-drying was greater for cells dried at the higher platen temperature and was influenced by the composition of the menstruum in which the cells were dried. In model systems having protein-dominant isotherms, survival during subsequent storage depended greatly on relative humidity, with recovery highest at relative humidities below those corresponding to moisture contents at which a monomolecular layer is formed. In menstrua having a higher sugar content, survival was best at low relative humidities corresponding to a very low equilibrium moisture content in the model system used. Damage during storage tended to be a function of the composition of the gels in which the organisms were freeze-dried, and also depended greatly on the presence of air and on the relative humidity. The maximal percentage of damage usually occurred at the low relative humidities as storage time increased.
鼠伤寒沙门氏菌在压板温度为120°F（48.9°C）时能在冻干过程中存活，在160°F（82.6°C）时也能存活，不过存活程度要小得多。在这些温度下的存活程度取决于所采用的模型系统的组成。冻干后立即出现的损伤发生率，对于在较高压板温度下干燥的细胞更高，并且受细胞干燥所用培养基组成的影响。在具有以蛋白质为主的等温线的模型系统中，后续储存期间的存活很大程度上取决于相对湿度，在低于形成单分子层的水分含量对应的相对湿度下恢复率最高。在糖含量较高的培养基中，在对应于所用模型系统中非常低的平衡水分含量的低相对湿度下存活最佳。储存期间的损伤往往是生物体冻干所用凝胶组成的函数，并且也极大地取决于空气的存在和相对湿度。随着储存时间的增加，最大损伤百分比通常发生在低相对湿度下。
