Helinski D R, Herschman H R
J Bacteriol. 1967 Sep;94(3):700-6. doi: 10.1128/jb.94.3.700-706.1967.
The effect of two Rec(-) mutations (AB2463 and JC1553) on the ability of a cell to accept, maintain, and express the colicinogenic factors ColE(1), ColE(2), and ColV was examined in Escherichia coli. These mutations had no observable effect on the colicinogenic properties of the ColV factor, but prevented the spontaneous and induced production of colicins E(1) and E(2) which are determined by the ColE(1) and ColE(2) factors, respectively. The two Rec(-) mutations had no apparent effect on the ability of the cells to acquire, maintain, or transfer the ColE(1) and ColE(2) factors. These mutations did not affect the expression of immunity by any of the three Col factors. ColE(1) and ColE(2) were also shown to be indirectly induced by mating F(-) cells carrying these Col factors with ultraviolet-irradiated, non-colicinogenic, Hfr and F(+) cells. Indirect induction of colicin production occurred when either an irradiated F(+) Rec(+) or F(+) Rec(-) strain was employed as the donor strain.
在大肠杆菌中检测了两种Rec(-)突变（AB2463和JC1553）对细胞接受、维持和表达产大肠杆菌素因子ColE(1)、ColE(2)和ColV能力的影响。这些突变对ColV因子的产大肠杆菌素特性没有可观察到的影响，但分别阻止了由ColE(1)和ColE(2)因子决定的大肠杆菌素E(1)和E(2)的自发产生和诱导产生。这两种Rec(-)突变对细胞获取、维持或转移ColE(1)和ColE(2)因子的能力没有明显影响。这些突变不影响三种Col因子中任何一种的免疫表达。还表明，携带这些Col因子的F(-)细胞与经紫外线照射的、不产大肠杆菌素的Hfr和F(+)细胞交配可间接诱导ColE(1)和ColE(2)。当使用经照射的F(+) Rec(+)或F(+) Rec(-)菌株作为供体菌株时，会发生大肠杆菌素产生的间接诱导。
