C1hu对C2hu的液相破坏作用。II. C4ihu揭示C1hu对C2hu的特异性。
Fluid phase destruction of C2hu by C1hu. II. Unmasking by C4ihu of C1hu specificity for C2hu.
作者信息
Gigli I, Austen K F
出版信息
J Exp Med. 1969 Oct 1;130(4):833-46. doi: 10.1084/jem.130.4.833.
Abstract
It has been demonstrated that C1 isolated in the unactivated form fails to inactivate C4 or C2 in the fluid phase, while the activated molecule, C1 rapidly converts C4 to hemolytically inactive C4i, but does not efficiently inactivate C2. The production and presence of C4i now confers on C1 the ability to rapidly inactivate C2. After heating at 56 degrees C, so as to destroy the hemolytic activity, heat inactivated C1 is still capable of inactivating C4 but the presence of C4i no longer confers an ability to inactivate C2. Studies with the subunits of C1-C1q, C1r, C1s, indicate that the action of C1s on C2 can be inhibited by C1r and that this inhibition is reversed by the presence of homologous C4. These studies indicate that the interaction of C4i with a heat labile receptor conformation in C1 uncovers a masked specificity for C2.
摘要
已证明，以未活化形式分离的C1无法在液相中使C4或C2失活，而活化分子C1能迅速将C4转化为无溶血活性的C4i，但不能有效地使C2失活。C4i的产生和存在赋予了C1迅速使C2失活的能力。在56摄氏度加热以破坏溶血活性后，热灭活的C1仍能使C4失活，但C4i的存在不再赋予其使C2失活的能力。对C1的亚基C1q、C1r、C1s的研究表明，C1r可抑制C1s对C2的作用，并且同源C4的存在可逆转这种抑制作用。这些研究表明，C4i与C1中热不稳定的受体构象的相互作用揭示了对C2的一种隐蔽特异性。
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2
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J Immunol. 1964 Jan;92:108-17.
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