Loomis W F
J Bacteriol. 1969 Oct;100(1):417-22. doi: 10.1128/jb.100.1.417-422.1969.
The kinetics of accumulation of alkaline phosphatase activity were determined in cells of wild-type and morphologically aberrant mutant strains of Dictyostelium discoideum induced to develop synchronously on membrane supports. The enzyme specific activity increased slowly in wild-type cells until culmination when a dramatic rise in specific activity occurred. The patterns of accumulation in the mutant strains, as well as previous electrophoretic analysis, suggest that the two phases of accumulation may result from the synthesis of distinct isozymes. The rapid accumulation of alkaline phosphatase was found to require concomitant protein synthesis. Ribonucleic acid synthesis, on the other hand, could be inhibited during the 8 hr immediately preceding culmination without affecting the amount of enzyme accumulated. When ribonucleic acid synthesis was inhibited earlier in development, the accumulation of alkaline phosphatase was reduced. Comparison of these results with work on other developmentally controlled enzymes suggests that both transcriptional and translational control occurs during development of D. discoideum. The accumulation of alkaline phosphatase was shown to require specific cellular topography during culmination, suggesting that intercellular interactions which allow synthesis of alkaline phosphatase occur at that stage.
在膜支持物上诱导同步发育的盘基网柄菌野生型和形态异常突变株的细胞中，测定了碱性磷酸酶活性积累的动力学。在野生型细胞中，酶的比活性缓慢增加，直到发育成熟时比活性才急剧上升。突变株中的积累模式以及先前的电泳分析表明，积累的两个阶段可能是由不同同工酶的合成导致的。发现碱性磷酸酶的快速积累需要伴随蛋白质合成。另一方面，在发育成熟前8小时内抑制核糖核酸合成，并不影响积累的酶量。当在发育早期抑制核糖核酸合成时，碱性磷酸酶的积累减少。将这些结果与其他发育调控酶的研究工作进行比较表明，在盘基网柄菌发育过程中发生了转录和翻译控制。结果表明，碱性磷酸酶的积累在发育成熟时需要特定的细胞拓扑结构，这表明允许合成碱性磷酸酶的细胞间相互作用在该阶段发生。
