盘基网柄菌中β-葡萄糖苷酶的同工酶
Isozymes of beta-glucosidase in Dictyostelium discoideum.
作者信息
Coston M B, Loomis W F
出版信息
J Bacteriol. 1969 Dec;100(3):1208-17. doi: 10.1128/jb.100.3.1208-1217.1969.
Abstract
The activity of beta-glucosidase (EC 3.2.1.21) in extracts of Dictyostelium discoideum was investigated. The specific activity increased early in development, declined during pseudoplasmodium formation, and increased again during sorocarp formation. The beta-glucosidase which was present in growing amoebae and during the first stages of multicellular development was electrophoretically distinct from the enzyme which accumulated during the final stages of morphogenesis. Ribonucleic acid synthesis and protein synthesis during development were required for the accumulation of the later isozyme. Analysis of beta-glucosidase activity in a number of morphological mutants suggests that the enzyme which accumulates late in morphogenesis is developmentally controlled.
摘要
对盘基网柄菌提取物中的β-葡萄糖苷酶（EC 3.2.1.21）活性进行了研究。比活性在发育早期增加，在伪原质团形成期间下降，并在子实体形成期间再次增加。生长中的变形虫和多细胞发育初期存在的β-葡萄糖苷酶在电泳上与形态发生最后阶段积累的酶不同。后期同工酶的积累需要发育过程中的核糖核酸合成和蛋白质合成。对一些形态突变体中β-葡萄糖苷酶活性的分析表明，形态发生后期积累的酶受发育调控。
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