将氚标记的视黄醛部分掺入细菌视紫红质的活性位点赖氨酸残基中。
The incorporation of tritiated retinyl moiety into the active-site lysine residue of bacteriorhodopsin.
作者信息
Mullen E, Gore M G, Akhtar M
出版信息
Biochem J. 1979 Oct 1;183(1):175-8. doi: 10.1042/bj1830175.
Abstract
Purple membranes were isolated from Halobacterium halobium bleached and regenerated with all-trans-[15-3H]retinal. The incorporation of label was 1.2 mol of retinal/mol of bacterio-opsin. The [3H]retinyl-bacterio-opsin obtained from regeneration was hydrolysed to give tritiated retinyl-lysine, which, on hydrogenation to N-epsilon-perhydro[3H]retinyl-lysine and reaction with 1-fluoro-2,4-dinitrobenzene, gave bis-(2,4-dinitrophenyl)-N-epsilon-perhydro[3H]retinyl-lysine. This result confirmed that the retinyl moiety of the chromophore is attached to an epsilon-amino group of lysine.
摘要
从经漂白并用全反式-[15-³H]视黄醛再生的嗜盐菌中分离出紫色膜。标记物的掺入量为每摩尔细菌视紫红质含1.2摩尔视黄醛。从再生过程中获得的[³H]视黄醛-细菌视紫红质经水解得到氚化视黄醛赖氨酸，将其氢化生成N-ε-全氢[³H]视黄醛赖氨酸，并与1-氟-2,4-二硝基苯反应，得到双-(2,4-二硝基苯基)-N-ε-全氢[³H]视黄醛赖氨酸。该结果证实发色团的视黄醛部分连接在赖氨酸的ε-氨基上。
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