Sano M, Ohya K, Ito R
Biomed Mass Spectrom. 1979 Nov;6(11):467-71. doi: 10.1002/bms.1200061102.
The identification of carpronium chloride and a metabolite in human urine has been performed by means of field desorption mass spectrometry using deuterium labelling. Essential points in this study are the simultaneous administration of a deuterium labelled drug ([2H9]carpronium chloride), a purification procedure by ion pair extraction with an iodine reagent, and the use of paper electrophoresis to examine the degree of clean-up. The field desorption mass spectra of the purified extracts obtained from sample urine gave a characteristic pattern resulting from the carpronium cation (m/z 160, m/z 169) and a metabolite of the N-(3-carbohydroxypropyl)trimethyl ammonium cation (m/z 146, m/z 155).
利用氘标记，通过场解吸质谱法对人尿中的卡泊氯铵及其一种代谢物进行了鉴定。本研究的要点包括同时给予氘标记药物（[2H9]卡泊氯铵）、用碘试剂进行离子对萃取的纯化程序以及使用纸电泳来检测净化程度。从尿样中获得的纯化提取物的场解吸质谱给出了由卡泊氯铵阳离子（m/z 160、m/z 169）和N-(3-碳羟丙基)三甲基铵阳离子的一种代谢物（m/z 146、m/z 155）产生的特征图谱。
