Miyazaki H, Shirai E, Ishibashi M, Hosoi K, Shibata S, Iwanaga M
Biomed Mass Spectrom. 1978 Oct;5(10):559-65. doi: 10.1002/bms.1200051002.
A method is described for the microdetermination of berberine chloride in human urine by a field desorption mass spectrometry selected ion monitoring system using a deuterium labelled analogue of berberine chloride as an internal standard. Prior to the quantitation of berberine in human urine, the fundamental problems related to field desorption selected ion monitoring, such as quality of emitters, amounts of sample loading, and the programming rate of the emitter current, were statistically investigated in detail. Berberine chloride can be determined in a concentration of 10 ng ml-1 in human urine by the method described. The analytical results were compared with those from gas chromatography mass spectrometry selected ion monitoring in the chemical ionization mode suggesting that the reliability of field desorption selected ion monitoring may be almost equivalent to that of gas chromatography chemical ionization selected ion monitoring.
本文描述了一种采用以氘标记的氯化小檗碱类似物为内标的场解吸质谱选择离子监测系统，对人尿中氯化小檗碱进行微量测定的方法。在对人尿中的小檗碱进行定量之前，对与场解吸选择离子监测相关的基本问题，如发射体质量、进样量以及发射体电流的编程速率等，进行了详细的统计学研究。采用所述方法可测定人尿中浓度为10 ng/ml的氯化小檗碱。将分析结果与化学电离模式下气相色谱-质谱选择离子监测的结果进行了比较，结果表明场解吸选择离子监测的可靠性与气相色谱-化学电离选择离子监测的可靠性几乎相当。
