Stamatoyannopoulos G, Yoshida A
Science. 1969 Nov 21;166(3908):1005-6. doi: 10.1126/science.166.3908.1005.
The mechanism of alkali resistance in hemoglobin Rainier, an adult human hemoglobin variant (beta 145 tyrosinie --> histidinie), has been investigated. Alkali denaturation kinetics, electrophoretic and hybridization study. and ultracentrifuge analysis provided evidence for a monomeric beta Rainiier chain resisting denaturation at alkaline pH. These data provide evidence for a previously unrecognized effect of a single amino acid substitution, that is, the change of the alkali denaturation properties of monomneric chains.
已对成人血红蛋白变体（β145酪氨酸→组氨酸）——雷尼尔血红蛋白的抗碱机制进行了研究。碱变性动力学、电泳和杂交研究以及超速离心分析为单体β雷尼尔链在碱性pH值下抗变性提供了证据。这些数据为单个氨基酸取代的一种先前未被认识的效应提供了证据，即单体链碱变性特性的改变。
