Nielsen N C, Fleischer S
Science. 1969 Nov 21;166(3908):1017-9. doi: 10.1126/science.166.3908.1017.
The enizyme D(-)-beta-hydroxybutyrate dehydrogenase has been reported to occur in all mitochondria isolated from mammalian tissues and is especially high in liver. By contrast only very low concentration of the enzyme is detectable in mitachondria from bovine and sheep liver. The amount of the enzyme in mitochondria from other ruminant tissues such as kidney and heart are comparable with those from mammalian sources. The verv low activity of beta-hydroxybutyrate dehydrogenase is not referable to the iniability of substrates to penetrate milochondria or to decreased electron transfer activity but rather to a lack of the enzyne in ruminant liver mitochondria.
据报道，酶D(-)-β-羟基丁酸脱氢酶存在于从哺乳动物组织分离出的所有线粒体中，在肝脏中含量尤其高。相比之下，在牛和羊肝脏的线粒体中只能检测到极低浓度的该酶。来自其他反刍动物组织（如肾脏和心脏）的线粒体中该酶的含量与来自哺乳动物组织的相当。β-羟基丁酸脱氢酶的极低活性并非由于底物无法穿透线粒体或电子传递活性降低，而是由于反刍动物肝脏线粒体中缺乏该酶。
