大鼠脑中脱氧核糖核酸的定量测定。
Quantitative determination of deoxyribonucleic acid in rat brain.
作者信息
Penn N W, Suwalski R
出版信息
Biochem J. 1969 Nov;115(3):563-8. doi: 10.1042/bj1150563.
Abstract
- A procedure is given for spectrophotometric analysis of rat brain DNA after its resolution into component bases. Amounts of tissue in the range 50-100mg. can be used. 2. The amount of DNA obtained by the present method is 80% greater than that reported for rat brain by a previous procedure specific for DNA thymine. Identity of the material is established by the base ratios of purines and pyrimidines. The features responsible for the higher yield are the presence of dioxan during alkaline hydrolysis of tissue, the determination of the optimum concentration of potassium hydroxide in this step and omission of organic washes of the initial acid-precipitated residues. 3. The requirement for dioxan during alkaline hydrolysis suggests a possible association of brain DNA with lipid. The concentration of potassium hydroxide that gives maximum yield is 0.1m, indicating that there may be internucleotide linkages in this DNA that are more sensitive to alkali than those of liver or thymus DNA. 4. This procedure gives low yields of DNA from liver. It is not suitable for analysis of the DNA from this tissue.
摘要
- 给出了一种大鼠脑DNA在分解为组成碱基后进行分光光度分析的方法。可使用50 - 100毫克范围内的组织量。2. 用本方法获得的DNA量比先前一种针对DNA胸腺嘧啶的方法所报道的大鼠脑DNA量多80%。通过嘌呤和嘧啶的碱基比率确定该物质的同一性。产量较高的原因在于组织碱性水解过程中存在二氧六环、此步骤中氢氧化钾最佳浓度的测定以及省略了对初始酸沉淀残渣的有机洗涤。3. 碱性水解过程中二氧六环的需求表明脑DNA可能与脂质有关联。产生最大产量的氢氧化钾浓度为0.1摩尔/升，这表明该DNA中的核苷酸间连接可能比肝脏或胸腺DNA中的那些连接对碱更敏感。4. 此方法从肝脏中获得的DNA产量较低。它不适用于分析该组织的DNA。
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