5-羟甲基胞嘧啶在动物脱氧核糖核酸中的存在。
The presence of 5-hydroxymethylcytosine in animal deoxyribonucleic acid.
作者信息
Penn N W, Suwalski R, O'Riley C, Bojanowski K, Yura R
出版信息
Biochem J. 1972 Feb;126(4):781-90. doi: 10.1042/bj1260781.
Abstract
A method is given for small-scale preparation of DNA from 1.0-1.5g of adult rat tissues. The product from brain or liver is characterized by base ratios and phosphorus content which accord with reported values for rat tissue. It is reasonably free of RNA, protein and glycogen. It contains 5-hydroxymethylcytosine at a content of about 15% of the total cytosine bases present. 5-Hydroxymethylcytosine is also demonstrable in mouse and frog brain DNA and in the crude cytidylic acid fractions obtained from RNA hydrolysates of rat brain and liver. 5-Hydroxymethylcytosine is identified by paper chromatography, u.v. spectra in acid and alkaline solutions and by its conversion into 5-hydroxymethyluracil.
摘要
本文给出了一种从小鼠脑组织制备RNA的方法。该方法包括用含0.5%十二烷基硫酸钠的缓冲液匀浆脑组织，然后用热酚抽提，再用乙醇沉淀RNA。从脑、肝组织中提取的RNA产物，经碱基比例和磷含量分析，其结果与报道的大鼠组织RNA值相符。该RNA制品不含DNA、蛋白质和糖原。用大鼠肝RNA水解物制备的粗胞苷酸级分中也含有5-羟甲基胞嘧啶。5-羟甲基胞嘧啶是通过纸层析、酸碱溶液中的紫外光谱以及它转化为5-羟甲基尿嘧啶来鉴定的。
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本文引用的文献
1
Improved separation of sugars on the paper partition chromatogram.纸分配色谱图上糖的分离得到改善。
Biochem J. 1949;44(4):402-7. doi: 10.1042/bj0440402.
2
Chromatographic studies of nucleic acids; a technique for the identification and estimation of purine and pyrimidine bases, nucleosides and related substances.核酸的色谱研究；一种用于嘌呤和嘧啶碱基、核苷及相关物质的鉴定和定量的技术。
Biochem J. 1949;45(3):294-8. doi: 10.1042/bj0450294.
3
The composition of the deoxyribonucleic acid of salmon sperm.鲑鱼精子脱氧核糖核酸的组成
J Biol Chem. 1951 Sep;192(1):223-30.
4
The purine and pyrimidine composition of deoxypentose nucleic acids.脱氧戊糖核酸的嘌呤和嘧啶组成。
Biochem J. 1951 May;48(5):584-90. doi: 10.1042/bj0480584.
5
Detection of sugars on paper chromatograms.纸色谱上糖的检测
Nature. 1950 Sep 9;166(4219):444-5. doi: 10.1038/166444b0.
6
THE DETERMINATION OF DEOXYRIBONUCLEIC ACID AND OF CELL NUMBER IN BRAIN.大脑中脱氧核糖核酸及细胞数量的测定
J Neurochem. 1964 Jul;11:505-9. doi: 10.1111/j.1471-4159.1964.tb07499.x.
7
Separation of deoxyribonucleic acid from ribonucleic acid without the use of enzymes.不使用酶从核糖核酸中分离脱氧核糖核酸。
Biochim Biophys Acta. 1961 Feb 12;47:18-26. doi: 10.1016/0006-3002(61)90824-1.
8
Deoxyribonucleic acids of central nervous system, kidney, and spleen: a comparison of some chemical and physical properties.中枢神经系统、肾脏和脾脏的脱氧核糖核酸：一些化学和物理性质的比较。
J Neurochem. 1960 May;5:236-44. doi: 10.1111/j.1471-4159.1960.tb13361.x.
9
The origin of purine and pyrimidine deoxyribose in T6r+ bacteriophage.T6r+噬菌体中嘌呤和嘧啶脱氧核糖的起源
J Biol Chem. 1959 Feb;234(2):364-9.
10
The bases of the nucleic acids of some bacterial and animal viruses: the occurrence of 5-hydroxymethylcytosine.某些细菌和动物病毒核酸的碱基：5-羟甲基胞嘧啶的存在情况
Biochem J. 1953 Dec;55(5):774-82. doi: 10.1042/bj0550774.
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