Waterhouse C, Keilson J
J Clin Invest. 1969 Dec;48(12):2359-66. doi: 10.1172/JCI106202.
12 subjects have been studied after an overnight fast with trace amounts of pyruvate-3-(14)C and glucose-6-(14)C. Blood disappearance curves and incorporation of the pyruvate-3-(14)C label into blood glucose have been determined. By the use of transfer functions which allow processes with many different chemical steps to be examined as a unit, we have determined the per cent of pyruvate and presumably lactate which is regenerated into glucose. 8 of the 12 subjects showed that 7-23 mg/kg per hr are recycled, while 4 subjects fell well outside this range. Correlation of increased activity was not good with any demonstrated metabolic abnormality (diabetes or obesity), and it is suggested from clinical observation of the subjects that anxiety may play a role.
对12名受试者进行了研究，这些受试者在禁食过夜后摄入了微量的丙酮酸-3-(14)C和葡萄糖-6-(14)C。测定了血液消失曲线以及丙酮酸-3-(14)C标记物掺入血糖的情况。通过使用传递函数，这些函数能够将具有许多不同化学步骤的过程作为一个整体来研究，我们确定了再生为葡萄糖的丙酮酸以及推测的乳酸的百分比。12名受试者中有8名显示每小时有7 - 23毫克/千克被循环利用，而4名受试者的情况明显超出此范围。活动增加与任何已证实的代谢异常（糖尿病或肥胖）之间的相关性不佳，并且从对受试者的临床观察表明焦虑可能起了作用。
