硫酸原黄素引起核酸前体掺入海拉细胞的刺激作用。
Stimulation of incorporation of nucleic acid precursors into HeLa cells caused by proflavine.
作者信息
Watts J W
出版信息
Biochem J. 1969 Dec;115(4):823-9. doi: 10.1042/bj1150823.
Abstract
- The effect of proflavine and other acridines on the incorporation of precursors into the nucleic acids of HeLa cells was examined. 2. Relatively low concentrations (50mum) of proflavine completely inhibited incorporation of precursors into DNA, but allowed a small extent of incorporation into RNA. 3. Acridine-resistant incorporation into RNA was unaffected by actinomycin D at 2mug./ml. and persisted even at high concentrations (500mum) of many acridines. 4. A few combinations of acridine and precursor, notably 250mum-proflavine and [(14)C]adenine, caused a stimulation of incorporation. 5. The proflavine-stimulated incorporation was into alkali-stable di- and tri-nucleotides. 6. It was concluded that the effect was due to the preferential inhibition of degradation of a fraction of RNA that normally turned over, thus allowing small radioactive oligonucleotides to accumulate in the cells.
摘要
- 研究了硫酸原黄素和其他吖啶对前体掺入海拉细胞核酸的影响。2. 相对低浓度（50μM）的硫酸原黄素完全抑制前体掺入DNA，但允许少量掺入RNA。3. 吖啶抗性掺入RNA不受2μg/ml放线菌素D的影响，即使在许多吖啶的高浓度（500μM）下仍持续存在。4. 吖啶和前体的一些组合，特别是250μM硫酸原黄素和[（14）C]腺嘌呤，导致掺入增加。5. 硫酸原黄素刺激的掺入是进入碱稳定的二核苷酸和三核苷酸。6. 得出的结论是，这种效应是由于优先抑制了一部分正常周转的RNA的降解，从而使小的放射性寡核苷酸在细胞中积累。
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2
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3
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Biochem J. 1952 Jan;50(3):384-97. doi: 10.1042/bj0500384.
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