硫酸原黄素对海拉细胞的作用。
The effect of proflavine on HeLa cells.
作者信息
Watts J W, Davis M A
出版信息
Biochem J. 1966 Aug;100(2):467-72. doi: 10.1042/bj1000467.
Abstract
- The effect of proflavine on the metabolism of RNA, DNA and protein of HeLa cells was studied. 2. The synthesis of RNA, DNA and protein was progressively inhibited by concentrations of proflavine up to 43mum. 3. There was no simple relationship between the degrees of inhibition of synthesis of RNA, DNA and protein by increasing concentrations of proflavine: the synthesis of RNA was most readily inhibited, and the synthesis of protein was relatively insensitive. 4. A concentration of 22mum-proflavine inhibited synthesis of RNA and DNA and caused a progressive loss of RNA from both nucleus and cytoplasm without any accompanying loss of DNA or dry weight from the cells. 5. The rapidly labelled RNA in the nucleus was preferentially degraded and was not transferred in a stable form to the cytoplasm.
摘要
- 研究了硫酸原黄素对海拉细胞RNA、DNA和蛋白质代谢的影响。2. 浓度高达43μm的硫酸原黄素可逐渐抑制RNA、DNA和蛋白质的合成。3. 随着硫酸原黄素浓度的增加，RNA、DNA和蛋白质合成的抑制程度之间没有简单的关系：RNA的合成最容易受到抑制，而蛋白质的合成相对不敏感。4. 22μm的硫酸原黄素浓度抑制了RNA和DNA的合成，并导致细胞核和细胞质中的RNA逐渐丢失，而细胞中的DNA或干重没有任何相应的损失。5. 细胞核中快速标记的RNA优先降解，不会以稳定的形式转移到细胞质中。
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