Weinhold P A, Burkel W E, Fischer T V, Kahn R H
In Vitro. 1979 Dec;15(12):1023-31. doi: 10.1007/BF02619161.
The maintenance of adult rat lung explants in organ culture was assessed both morphologically and biochemically. A comparison of several culture media indicated that Ham's F12K plus 1.0 microM dexamethasone, which maintained the explants for 14 days, was superior. The ability of the explants to synthesize dipalmitoylphosphatidylcholine increased with the length of cultivation to values greater than the noncultivated controls. The DNA content remained constant for 7 days, and a relatively normal structural relationship between type I and type II pneumocytes was maintained. Explants cultivated in Ham's F12K without dexamethasone did not maintain a histotypic morphology; the type II pneumocytes appeared to proliferate and the ability to synthesize phosphatidylcholine decreased.
通过形态学和生物化学方法评估成年大鼠肺外植体在器官培养中的维持情况。对几种培养基的比较表明，添加1.0微摩尔地塞米松的Ham's F12K培养基能将外植体维持14天，效果更佳。外植体合成二棕榈酰磷脂酰胆碱的能力随培养时间延长而增加，达到高于未培养对照组的值。DNA含量在7天内保持恒定，I型和II型肺上皮细胞之间维持着相对正常的结构关系。在不添加地塞米松的Ham's F12K培养基中培养的外植体无法维持组织型形态；II型肺上皮细胞似乎增殖，合成磷脂酰胆碱的能力下降。
