Greenberg M S, Mayer W J
J Chromatogr. 1979 Feb 1;169:321-7. doi: 10.1016/0021-9673(75)85056-4.
Theophylline and its derivatives are determined by high-performance liquid chromatographic separation on a C8 reversed-phase column with electrochemical detection. The analyte is oxidized at 1.24 V (vs. the standard calomel electrode) on a wax-impregnated graphite electrode using classical d.c. and current sampled d.c. modes, and the results obtained compared with those of a 254 nm ultraviolet (UV) detector. Polarographic detection offers greater sensitivity for theophylline than UV detection; further, interferences which hamper UV detection may be selectively eliminated with polarographic detection.
采用C8反相柱进行高效液相色谱分离，并用电化学检测法测定茶碱及其衍生物。使用经典直流和电流采样直流模式，在涂蜡石墨电极上，分析物在1.24 V（相对于标准甘汞电极）下被氧化，并将所得结果与254 nm紫外（UV）检测器的结果进行比较。极谱检测对茶碱的灵敏度高于紫外检测；此外，极谱检测可选择性地消除妨碍紫外检测的干扰。
