Stulík K, Pacáková V
J Chromatogr. 1983 Mar 11;273(1):77-86.
High-performance liquid chromatographic separation of a number of biologically important pyrimidine derivatives was studied in the reversed-phase system. Good results were obtained using a C18 alkyl-bonded silica column and an aqueous citrate--phosphate mobile phase of pH 3.5. All eluted components are detected with the UV absorbance detector at 254 nm, whereas the voltammetric detector with a polymeric carbon-paste electrode detects only derivatives containing oxidizable functional groups (amino, mercapto) and the polarographic detector with a mercury electrode only those with reducible groups (nitro, aza). The signal of the electrochemical detectors is proportional to the number of electroactive groups in the solute molecule. The use of two or three detectors in series thus improves the resolution of complex mixtures and facilitates identification.
在反相系统中研究了多种具有生物学重要性的嘧啶衍生物的高效液相色谱分离。使用C18烷基键合硅胶柱和pH值为3.5的柠檬酸 - 磷酸盐水性流动相可获得良好结果。所有洗脱组分均用254nm的紫外吸收检测器检测，而使用聚合物碳糊电极的伏安检测器仅检测含有可氧化官能团（氨基、巯基）的衍生物，使用汞电极的极谱检测器仅检测含有可还原基团（硝基、氮杂）的衍生物。电化学检测器的信号与溶质分子中电活性基团的数量成正比。因此，串联使用两个或三个检测器可提高复杂混合物的分离度并便于鉴定。
