Verbeke R
J Chromatogr. 1979 Sep 14;177(1):69-84. doi: 10.1016/s0021-9673(00)92600-1.
A routine procedure is described for the dependable detection of various anabolic residues in tissues or urine contaminated at levels as low as 0.5-10 ppb (10 parts per 10(9)). A suitable extraction and clean-up procedure was developed, permitting adequate recovery (60-80%) of various anabolics from tissue samples (50 g) or urine (50 ml). Following two-dimensional thin-layer chromatography, the presence of the anabolic residues are detected by sulphuric acid-induced fluorescence. The detection limit of most anabolics is of the order of 1-10 ng.
本文描述了一种常规方法，用于可靠地检测组织或尿液中低至0.5-10 ppb（十亿分之十）水平污染的各种合成代谢残留物。开发了一种合适的提取和净化程序，可从组织样本（50克）或尿液（50毫升）中充分回收（60-80%）各种合成代谢物。经过二维薄层色谱法后，通过硫酸诱导荧光检测合成代谢残留物的存在。大多数合成代谢物的检测限约为1-10纳克。
