Impens Sandra, De Wasch Katia, Comelis Marc, De Brabander Hubert F
Department of Veterinary Food Inspection, Faculty of Veterinary Medicine of Ghent University, Merelbeke, Belgium.
J Chromatogr A. 2002 Sep 13;970(1-2):235-47. doi: 10.1016/s0021-9673(02)00313-8.
The use of estrogens, gestagens and androgens (EGAs) in animal fattening is prohibited in the European Community. Based on the general detection capabilities of Belgian laboratories, National Minimum Required Performance Limits (National MRPLs) for a number of EGAs have been imposed by the inspection services. Selective hyphenated techniques, e.g. GC-MS and GC-MS2, with high detection capability are needed. Beta-trenbolone, which is meant to be a "problem" molecule for GC-MS, can be detected at the 2 microg/kg level using GC-MS2. Based on the National MRPLs in different matrices, our laboratory has divided the EGAs into a class system. In this set-up, analysis of EGAs in kidney fat and meat is discussed.
在欧洲共同体，禁止在动物育肥过程中使用雌激素、孕激素和雄激素（EGAs）。根据比利时实验室的一般检测能力，检查服务部门规定了多种EGAs的国家最低要求性能限值（国家MRPLs）。需要采用具有高检测能力的选择性联用技术，例如气相色谱 - 质谱联用（GC-MS）和二级气相色谱 - 质谱联用（GC-MS2）。β-群勃龙，这对GC-MS来说本应是一种“难测”分子，使用GC-MS2可在2微克/千克的水平进行检测。基于不同基质中的国家MRPLs，我们实验室已将EGAs划分为一个类别系统。在此设置下，讨论了肾周脂肪和肉类中EGAs的分析。
